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讲师:薛升

2016年09月27日 15:47  点击:[]

薛升,男,1984年出生,博士,讲师。

2014年毕业于华中农业大学,毕业后获得CSC-DAAD博士后奖学金,赴德国Fraunhofer Institute for Molecular Biology and Applied Ecology IME研究所从事博士后阶段研究;2016年进入青岛大学医学部工作,博士和博士后研究期间开展了黄曲霉特异性单克隆抗体和单链抗体研究。构建了鸡源单链抗体(scFv)噬菌体展示文库,筛选到了多个高亲和力黄曲霉菌特异性单链抗体,并利用单链抗体融合蛋白建立了高效黄曲霉菌酶联免疫检测技术。

研究方向

1.噬菌体展示技术研究

2.基因工程抗体研究

代表论文:(#第一作者,*通讯作者)

1. Sheng Xue#, Heping Li, Jingbo Zhang, Jinlong Liu, Zuquan Hu, AndongGong, Tao Huang, Yucai Liao*, Chicken single-chain antibody fused to alkaline phosphatase detectsAspergilluspathogens and their presence in natural samples by direct sandwich enzyme-linked immunosorbent assay, Analytical Chemistry, 2013, 85:10992-10999.

2. Xiushi Song, Shu Xing, Heping Li, Jingbo Zhang, Bo Qu, Jinhe Jiang, Chao Fan, Peng Yang, Jinglong Liu, Zuquan Hu,Sheng Xue, Yucai Liao*, An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase inFusarium, New Phytologist, 2015, 210.

3. Jianhua Wang, Jingbo Zhang, Heping Li, Andong Gong,Sheng Xue, Rebecca S. Agboola, Yucai Liao*, Molecular Identification, Mycotoxin Production and Comparative Pathogenicity ofFusarium temperatumIsolated from Maize in China, Journal of Phytopathology, 2013, 162(3): 147-157.

4. Zuquan Hu, Heping Li, Jingbo Zhang, Tao Huang, Jinlong Liu,Sheng Xue, Aibo Wu, Yucai Liao*, A phage-displayed chicken single-chain antibody fused to alkaline phosphatase detectsFusariumpathogens and their presence in cereal grains, Analytica Chimica Acta, 2013, 764:84-92.

5. Zuquan Hu, Jinlong Liu, Heping Li, Shu Xing,Sheng Xue, Jingbo Zhang, Jianhua Wang, Greta Nölke, Yucai Liao*, Generation of a highly reactive chicken-derived single-chain variable fragment againstFusarium verticillioidesby phage display, International Journal of Molecular Sciences, 2012, 13(6): 7038-7056.

6. Jinlong Liu, Huzu Quan, Shu Xing, Sheng Xue, Heping Li, Jingbo Zhang, Yucai Liao*, Attainment of 15-Fold Higher Affinity of aFusarium-specific Single-Chain Antibody by Directed Molecular Evolution Coupled to Phage Display, Molecular Biotechnology, 2012, 52(2): 111-122.

专利:

廖玉才,薛升,李和平,黄曲霉特异性单链抗体的制备方法和应用,2015.09.10,中国,ZL201310477107.0(已授权)

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